Conference Proceeding

Author(s): Pradeep R, Sherin Swathi Juliet Christopher, Malini Babu, Gopalakrishnan Menon

Email(s): m.gopalakrishnan@jainuniversity.ac.in

Address: Pradeep R, Sherin Swathi Juliet Christopher, Malini Babu, Gopalakrishnan Menon*
Department of Microbiology and Botany, School of Sciences, Jain (Deemed to be) University, 1st Cross JC Road, Bengaluru - 560027, Karnataka, India
*Corresponding Author

Published In:   Conference Proceeding, Proceeding of ICONS-2024

Year of Publication:  July, 2025

Online since:  July 11, 2025

DOI: Not Available

ABSTRACT:
Cellulase, a complex enzyme made up of endoglucanase, exoglucanase, and beta-glucosidase, hydrolyzes cellulose into glucose units. Different microorganisms manufacture the enzyme. Most individuals use fungal cellulase than bacterial cellulase. It is because of their high enzyme productivity. Aspergillus and Trichoderma are the most significant fungi that produce cellulase during solid-state fermentation (SSF). Among these, Trichoderma is a commonly used organism that produces significant numbers of cellulase and hemicellulase, which are essential for the breakdown of lignocellulose. The enzyme's ability to break down lignocellulosic biomass makes it possible to manufacture bioethanol and other second-generation biofuels. As a result of global problems like energy shortages and the increasing need for bio-based solutions, Trichoderma has evolved from traditional mutagenesis to state-of-the-art molecular biology. Today, Trichoderma leads the way in enzyme production, contributing to a wide range of industrial uses. Using market effluents and agricultural wastes as precursors, the study aims to manufacture cellulase enzymes from Trichoderma. Additionally, it aims to optimize the physiochemical parameters and enhance the strain for optimal enzyme synthesis. Today, Trichoderma leads the way in enzyme production, contributing to a wide range of industrial uses. Using market effluents and agricultural wastes as precursors, the study aims to manufacture cellulase enzyme from Trichoderma. Additionally, it aims to optimize the physiochemical parameters and enhance the strain for optimal enzyme synthesis.


Cite this article:
Pradeep R, Sherin Swathi Juliet Christopher, Malini Babu, Gopalakrishnan Menon. Optimization of culture conditions and strain improvement for maximum Cellulase production by Trichoderma. Proceeding of ICONS-2024. 79-84.


REFERENCES:

1.      Fanglin Zheng, Renfei Yang, Yanli Cao, Weixin Zhang, Xinxing Lv, Xiangfeng Meng, Yaohua Zhong, Guanjun Chen, Qingxin Zhou, and Weifeng Liu, Engineering Trichoderma reesei for Hyperproduction of Cellulases on Glucose to Efficiently Saccharify Pretreated Corncobs V, [2020], Journal of Agricultural and Food Chemistry, 68(45):12671-12682.

2.      V. Anoop Kumar, R. Suresh Chandra Kurup, C. Snishamol and G. Nagendra Prabhu, Role of Cellulases in Food, Feed, and Beverage Industries, [2019], Green Bio-processes (pp.323-343)

3.      Nikita Bhati, Shreya, Arun Kumar Sharma, Cost-effective cellulase production, improvement strategies, and future challenges, [2020], Journal of Food Process Engineering 44(3)

4.      Gamachis Korsa, Rocktotpal Konwarh, Chandran Masi, Abate Ayele and Setegn Haile, Microbial cellulase production and its potential application for textile industries, [2023], Annals of Microbiology (73:13)

5.      Kamaluddeen, S.Y. and Madika, A, Production of Cellulase by Trichoderma viride via Submerged Fermentation using Corncob as Substrate [2023], Nigerian Journal of Pure and Applied Sciences, Vol. 36 (1): 4512

6.      Wang, J. Sh., Wang, J., and Gulfraz, M. "Efficient Cellulase Production from Corn Straw by Trichoderma Reesei LW1 Through Solid State Fermentation Process", [2005] Ethnobotanical Leaflets: Vol. 2005: Iss. 1, Article 7.

7.      P.Elakkiya and V.Muralikrishnan, Cellulase production and purification of mutant strain Trichoderma viride, International Journal of Current Microbiology and Applied Sciences (IJCMAS) [2014], ISSN: 2319-7706 Volume 3 Number 9, pp. 720-727

8.      Alan O. Campos, Estéfani A. Asevedo, Pedro F. Souza Filho  and Everaldo S. dos Santos , Extraction of Cellulases Produced through Solid-State Fermentation by Trichoderma reesei CCT-2768 Using Green Coconut Fibers Pretreated by Steam Explosion Combined with Alkali, Biomass [2024], 4, 92–106.

9.      D. S. Chahal, Solid-State Fermentation with Trichoderma reesei for Cellulase Production, Applied and Environmental Microbiology [1985], Volume 49; 205-210.

 

 

 





Author/Editor Information

Dr. Vani. R

Professor

Dr. Apurva Kumar R. Joshi

Assistant Professor and Program Head